Publications

  • Benhar, M. Application of a thioredoxin-trapping mutant for analysis of the cellular nitrosoproteome. Methods Enzymol. 585:285-294, 2017.
  • Ben-Lulu, S., Ziv, T., Weisman-Shomer, P. and Benhar, M. Nitrosothiol-trapping-based proteomic analysis of S-nitrosylation in human lung carcinoma cells. PLoS One. 12(1):e0169862, 2017.
  • Engelman, R., Ziv, T., Arner, E.S. and Benhar, M. Inhibitory nitrosylation of mammalian thioredoxin reductase 1: molecular characterization and evidence for its functional role in cellular nitroso-redox imbalance. Free Radic. Biol. Med. 97:375-85, 2016.
  • Benhar, M. Emerging roles of S-nitrosylation in macrophages and cancer cells: insights from proteomic studies. Current Medicinal Chemistry. 23:2602-17, 2016.
  • Benhar, M.*, Shytaj, I.L.*, Stamler, J.S. and Savarino, ADual targeting of the thioredoxin and glutathione systems in cancer and HIV. J. Clin. Invest. 126:1630-1639, 2016.
  • Benhar, M. (2015) Nitric oxide and the thioredoxin system: a complex interplay in redox regulation. Biochim. Biophys. Acta. 1850:2476-2484.
  • Voloshin, T., Alishkevitz, D., Kaneti, L., Miller, V., Isakov, E., Kaplanov, I., Voronov, E., Fremder, E., Benhar, M., Machluf, M., Apte, R.N., Shaked, Y. (2015) Blocking IL-1β pathway following paclitaxel chemotherapy slightly inhibits primary tumor growth but promotes spontaneous metastasis. Mol. Cancer Ther. 14:1385-942015.
  • Kronenfeld, G., Engelman, R., Weisman-Shomer, P., Atlas, D. and Benhar, M. (2015) Thioredoxin-mimetic peptides as catalysts of S-denitrosylation and anti-nitrosative stress agents. Free Radic. Biol. Med. 50:138-46.
  • Isakov, E., Weisman-Shomer, P. and Benhar, M. (2014) Suppression of the pro-inflammatory NLRP3/interleukin-1β pathway in macrophages by the thioredoxin reductase inhibitor auranofin. Biochim. Biophys. Acta. 1840(10):3153-61.
  • Ben-Lulu, S., Ziv, T., Admon, A., Weisman-Shomer, P. and Benhar, M. (2014) A substrate trapping approach identifies proteins regulated by reversible S-nitrosylation. Mol. Cell. Proteomics. 13(10):2573-83.
  • Hertz, R., Ben Lulu, S., Shahi, P., Geffen, M., Benhar, M. and Ankri, S. (2014) Proteomic identification of S-nitrosylated proteins in the parasite Entamoeba histolytica by resin-assisted capture: insights into the regulation of the Gal/GalNAc Lectin by nitric oxide. PLoS One. 9(3):e91518.
  • Engelman, R., Weisman-Shomer, P., Ziv, T., Xu, J., Arner, E.S. and Benhar, M. (2013) Multilevel Regulation of 2-Cys Peroxiredoxin Reaction Cycle by S-Nitrosylation. J. Biol. Chem. 288:11312-24.
  • Cohen-Kutner M, Khomsky L, Trus M, Aisner Y, Niv MY, Benhar M. and Atlas D. (2013) Thioredoxin-mimetic peptides (TXM) reverse auranofin induced apoptosis and restore insulin secretion in insulinoma cells. Biochem Pharmacol. 85:977-90.
  • Ovadia, H., Haim, Y., Nov, O., Almog, O., Kovsan, J., Bashan, N., Benhar, M. and Rudich, A. (2011) Increased adipocyte s-nitrosylation targets the anti-lipolytic action of insulin: relevance to adipose tissue dysfunction in obesity. J. Biol. Chem. 286:30433-43.
  • Benhar, M. and Schonhoff, C.M. Analysis of Protein S-Nitrosylation. (2011) Curr. Prot. Prot. Sci. Chapter 14:Unit14.6.
  • Benhar, M., Thompson, J.W., Moseley, M.A. and Stamler, J.S. (2010) Identification of S-nitrosylated targets of thioredoxin using a quantitative proteomic approach. Biochemistry. 49:6963-9.
  • Forrester, M.T., Seth, D., Hausladen, A., Eyler, C.E., Foster, M.W., Matsumoto, A., Benhar, M., Marshall, H.E. and Stamler, J.S. (2009) Thioredoxin interacting protein (Txnip) is a feedback regulator of S-nitrosylation. J. Biol. Chem. 284:36160-6.
  • Benhar, M., Forrester, M.T. and Stamler, J.S.  (2009) Protein denitrosylation: enzymatic mechanisms and cellular functions. Nature Rev. Mol. Cell Biol. 10:721-32.
  • Forrester, M.T., Foster, M.W., Benhar, M. and Stamler, J.S. (2008) Detection of protein S-nitrosylation with the biotin-switch technique. Free Radic. Biol. Med. 46:119-126.
  • Benhar, M., Forrester, M.T., Hess, D.T. and Stamler, J.S. (2008) Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins. Science. 320:1050-4.
  • Whalen, E.J., Foster, M.W., Matsumoto, A., Ozawa, K., Violin, J.D., Que, L.G., Nelson, C.D., Benhar, M., Keys, J.R., Rockman, H.A., Koch, W.J., Daaka, Y., Lefkowitz, R.J. and Stamler, J.S. (2007) Regulation of beta-adrenergic receptor signaling by S-nitrosylation of G-protein-coupled receptor kinase 2. Cell. 129:511-22.
  • Forrester, M.T., Benhar, M. and Stamler, J.S. (2006) Nitrosative stress in the ER: A new role for S-nitrosylation in neurodegenerative diseases. ACS Chem. Biol. 1:355-8.
  • Benhar, M. and  Stamler, J.S. (2005) A central role for S-nitrosylation in apoptosis. Nature Cell Biol. 7:645-6.
  • Offen, D., Gilgun-Sherki, Y., Barhum, Y., Benhar, M., Grinberg, L., Reich, R., Melamed, E. and Atlas, D. (2004) A low molecular weight copper chelator crosses the blood-brain barrier and attenuates experimental autoimmune encephalomyelitis. J. Neurochem. 89:1241-51.
  • Benhar, M., Engelberg, D. and Levitzki, A. (2002) Cisplatin-induced activation of the EGF Receptor. Oncogene. 21:8723-31.
  • Reuveni, H., Livnah, N., Geiger, T., Klein, S., Ohne, O., Cohen, I., Benhar, M., Gellerman, G. and Levitzki, A. (2002) Toward a PKB inhibitor: modification of a selective PKA inhibitor by rational design. Biochemistry. 41:10304-14
  • Benhar, M., Engelberg, D. and Levitzki, A. (2002) ROS, stress-kinases and stress signaling in cancer. EMBO Rep. 3:420-5.
  • Benhar, M., Dalyot, I., Engelberg, D. and Levitzki, A. (2001) Enhanced ROS production in oncogenically transformed tells potentiates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation and sensitization to genotoxic stress. Mol. Cell. Biol. 21:6913-26.
  • Benhar, M., Boschwitz, H. and Linial, M. (1997) Differential expression pattern of Rab-GDI isoforms during the parotid gland secretion cycle. Exp. Cell Res. 233:207-15.